Screening for antimicrobial residues in poultry eggs in Bangladesh using Charm II radio-receptor assay technique following validation.

The aim of the study was to screen for the presence of antimicrobial residues in poultry eggs from Bangladesh using the Charm II radio-receptor assay in the absence of expensive confirmatory instrumentation. This was based on cut-off values as set in the validation guidelines according to Commission Decision 2002/657/EC and Commission Implementing Regulation (EU) 2021/808. Fortified eggs spiked with fixed concentrations of doxycycline, erythromycin A, sulphamethazine, and benzylpenicillin were used to determine the cut-off values and detection capabilities (CCß). Other validation parameters included were applicability, ruggedness, and robustness. A total of 201 egg mix samples from native organic chicken, duck, and commercial farm-raised laying hens (both brown and white eggs) were tested and after analysis 13%, 10%, and 4.5% of the egg mix samples showed positive signals for sulphonamides, macrolides/lincosamides, and tetracyclines, respectively. Presence of multiple drug residues were also suspected in 11 out of 201 egg mix samples.

Macroalgae for biomonitoring of trace elements in relation to environmental parameters and seasonality in a sub-tropical mangrove estuary.

Being a resourceful ecosystem, mangrove estuaries have always been subjected to trace elements (TEs) contamination, and therefore, the biomonitoring approach holds immense potential for surveilling the aquatic environment. To investigate the potentiality of mangrove macroalgae as biomonitors, estuarine water, intertidal-sediment, and macroalgal samples were collected from the Pasur River estuary of Sundarbans mangrove ecosystem, Bangladesh, and afterward studied through Atomic Absorption Spectrometer to quantify the levels of six concerned TEs (Fe, Mn, Zn, Cu, Pb, and Cd). This study utilized the geo-environmental and ecological indices and sediment characterization approaches (sediment quality guidelines-SQGs) for assessing the contamination scenario of the adjacent environment to macroalgae whereas the performance of studied algal groups was evaluated using Bio-contamination factor, Comprehensive bio-concentration index, and Metal accumulation index. Metal occurrence scheme in the water followed the order of Fe > Zn > Mn > Pb > Cd while Fe > Mn > Zn > Cu > Pb > Cd for both sediment and macroalgae. Both Pb and Cd exceeded the guideline limit in estuarine water and the indices approach manifested low to moderate contamination with enrichment from anthropogenic origin of Mn, Zn, and Cu in sediment. Moreover, the SQGs revealed rare biological effects of Cu on an aquatic community. Within algal samples, Chlorophyta contributed the highest biomass production, followed by Phaeophyta and Rhodophyta. Statistical relationship disclosed the influence of environmental variables on TE's accumulation in Chlorophyta. By contrast, hydrochemical's association showed prevalence over the TEs accumulation process for Phaeophyta and Rhodophyta. Bioaccumulation performance analysis revealed that the ability to accumulate TEs in macroalgal groups varied with seasons. Therefore, biomonitoring with macroalgae for the region of interest might require further temporal considerations.

Retracted Human Health Risk of Chromium Intake From Consumption of Poultry Meat and Eggs in Dhaka, Bangladesh.

BACKGROUND: Heavy metals contamination of food is a serious threat. Long term exposure may lead to human health risks. Poultry and eggs are a major source of protein, but if contaminated by heavy metals, have the potential to lead to detrimental effects on human health. OBJECTIVES: The objective of this study is to determine chromium concentrations in poultry meat (flesh and liver) and eggs collected from poultry farms in Dhaka, Bangladesh, to calculate the daily intake of chromium from the consumption of poultry meat and eggs for adults, and to evaluate their potential health risk by calculating the target hazard quotients (THQ). METHODS: All samples of poultry feed, meat (flesh and liver) and eggs were analyzed by a graphite furnace atomic absorption spectrometer (AAS) (GFA- EX- 7i Shimadju, Japan). RESULTS: Chromium concentrations were recorded in the range of not detected (ND) to 1.3926±0.0010 mg kg-1 and 0.0678±0.0001 mg kg-1 to 1.3764±0.0009 mg kg-1 in the liver of broiler and layer chickens, respectively. Chromium concentrations were determined in the range of 0.069±1.0004 mgkg-1 to 2.0746±0.0021 mg kg-1 and 0.0362±0.0002 mg kg-1 to 1.2752±0.0014 mg kg-1 in the flesh of broiler and layer chicken, respectively. The mean concentration of chromium in eggs was 0.2174-1.08 mg kg.-1 The highest concentration of chromium 2.4196±0.0019 mg kg-1 was found in egg yolk. Target hazard quotients values in all poultry flesh, liver and eggs samples were less than one, indicating no potential health risks to consumers. CONCLUSIONS: The estimated daily intake values of chromium were below the threshold limit. Thus, our results indicate that no adverse health effects are expected as a resultof ingestion of chicken fed with tannery waste. ETHICS APPROVAL: This study was approved by the Biosafety, Biosecurity & Ethical Committee of Jahangirnagar University.

Circadian regulation of olfactory receptor neurons in the cockroach antenna.

In the cockroach, olfactory sensitivity as measured by the amplitude of the electroantennogram (EAG) is regulated by the circadian system. We wished to determine how this rhythm in antennal response was reflected in the activity of individual olfactory receptor neurons. The amplitude of the EAG and the activity of olfactory receptor neurons (ORNs) in single olfactory sensilla were recorded simultaneously for 3 to 5 days in constant darkness from an antenna of the cockroach Leucophaea maderae. Both EAG amplitude and the spike frequency of the ORNs exhibited circadian rhythms with peak amplitude/activity occurring in the subjective day. The phases of the rhythms were dependent on the phase of the prior light cycle and thus were entrainable by light. Ablation of the optic lobes abolished the rhythm in EAG amplitude as has been previously reported. In contrast, the rhythm in ORN response persisted following surgery. These results indicated that a circadian clock outside the optic lobes can regulate the responses of olfactory receptor neurons and further that this modulation of the ORN response is not dependent on the circadian rhythm in EAG amplitude.

Characterization of PDF-immunoreactive neurons in the optic lobe and cerebral lobe of the cricket, Gryllus bimaculatus.

Pigment-dispersing factor (PDF) is a neuropeptide playing important roles in insect circadian systems. In this study, we morphologically and physiologically characterized PDF-immunoreactive neurons in the optic lobe and the brain of the cricket Gryllus bimaculatus. PDF-immunoreactivity was detected in cells located in the proximal medulla (PDFMe cells) and those in the dorsal and ventral regions of the outer chiasma (PDFLa cells). The PDFMe cells had varicose processes spread over the frontal surface of the medulla and the PDFLa cells had varicose mesh-like innervations in almost whole lamina, suggesting their modulatory role in the optic lobe. Some of PDFMe cells had a hairpin-shaped axonal process running toward the lamina then turning back to project into the brain where they terminated at various protocerebral areas. The PDFMe cells had a low frequency spontaneous spike activity that was higher during the night and was often slightly increased by light pulses. Six pairs of PDF-immunoreactive neurons were also found in the frontal ganglion. Competitive ELISA with anti-PDF antibodies revealed daily cycling of PDF both in the optic lobe and cerebral lobe with an increase during the night that persisted in constant darkness. The physiological role of PDF is discussed based on these results.

Phase shifts of the circadian locomotor rhythm induced by pigment-dispersing factor in the cricket Gryllus bimaculatus.

Pigment-dispersing factors (PDFs) are octadeca-peptides widely distributed in insect optic lobes and brain. In this study, we have purified PDF and determined its amino acid sequence in the cricket Gryllus bimaculatus. Its primary structure was NSEIINSLLGLPKVLNDA-NH(2), homologous to other PDH family members so far reported. When injected into the optic lobe of experimentally blinded adult male crickets, Gryllus-PDF induced phase shifts in their activity rhythms in a phase dependent and dose dependent manner. The resulted phase response curve (PRC) showed delays during the late subjective night to early subjective day and advances during the mid subjective day to mid subjective night. The PRC was different in shape from those for light, serotonin and temperature. These results suggest that PDF plays a role in phase regulation of the circadian clock through a separate pathway from those of other known phase regulating agents.

Pigment-dispersing factor sets the night state of the medulla bilateral neurons in the optic lobe of the cricket, Gryllus bimaculatus.

Pigment-dispersing factor (PDF) is an octadeca-neuropeptide widely distributed in the insect brain and suggested to be involved in the insect circadian systems. We have examined its effects on the neuronal activity of the brain efferents in the optic stalk including medulla bilateral neurons (MBNs) in the cricket, Gryllus bimaculatus. The MBNs are visually responding interneurons connecting the bilateral medulla, which show a clear day/night change in their light responsiveness that is greater during the night. Microinjection of PDF into the optic lobe induced a significant increase in the spontaneous activity of the brain efferents and the photo-responsiveness of the MBNs during the day, while little change was induced during the night. The enhancing effects began to occur about 20 min after the injection and another 10 min was necessary to reach the maximal level. The effects of PDF were dose-dependent. When 22 nl of anti-Gryllus-PDF (1:200) IgG was injected into the medulla, the photo-responsiveness of the MBNs was suppressed in both the day and the night with greater magnitude during the night. No significant suppression was induced by injection of the same amount of IgG from normal rabbit serum. These results suggest that in the cricket optic lobe, PDF is released during the night and enhances MBNs' photo-responsiveness to set their night state.

5-HT(7)-like receptors mediate serotonergic modulation of photo-responsiveness of the medulla bilateral neurons in the cricket, Gryllus bimaculatus.

Serotonin (5-HT) suppresses the photo-responsiveness of medulla bilateral neurons (MBNs) that are involved in the coupling mechanism of the bilaterally paired optic lobe circadian pacemakers in the cricket, Gryllus bimaculatus. We found that forskolin, a highly specific activator of adenylate cyclase, mimicked the effects of serotonin on the MBNs. This fact suggests the involvement of cyclic 3', 5'-adenosine monophosphate (cAMP) in mediating the action of serotonin. We therefore tested the effects of various 5-HT receptor agonists and antagonists that are coupled to adenylate cyclase to specify the receptor involved. Application of 8-OH-DPAT that has affinity for both 5-HT(1A) and 5-HT(7) receptors suppressed the photo-responsiveness, like forskolin. The inhibitory effect of 8-OH-DPAT was effectively blocked by clozapine, a high affinity 5-HT(7) receptor antagonists with a very low affinity for 5-HT(2). Ketanserin, a selective 5-HT(2) antagonist, and NAN-190, a 5-HT(1A) antagonist, did not block it. These results suggest that serotonergic suppression of the photo-responsiveness of the MBNs is mediated by 5-HT(7)-like receptor subtypes.

Serotonin sets the day state in the neurons that control coupling between the optic lobe circadian pacemakers in the cricket Gryllus bimaculatus.

The bilaterally paired optic lobe circadian pacemakers of the cricket Gryllus bimaculatus mutually exchange photic and circadian information to keep their activity synchronized. The information is mediated by a neural pathway, consisting of the so-called medulla bilateral neurons, connecting the medulla areas of the two optic lobes. We investigated the effects of serotonin on the neural activity in this coupling pathway. Spontaneous and light-induced electrical activity of the neurons in the coupling pathway showed daily variations, being more intense during the night than the day. Microinjection of serotonin or a serotonin-receptor agonist, quipazine, into the optic lobe caused a dose- and time-dependent inhibition of spontaneous and light-induced responses, mimicking the day state. The amount of suppression was greater and the recovery from the suppression occurred faster during the night. Application of metergoline, a non-selective serotonin-receptor antagonist, increased spontaneous activity and light-evoked responses during both the day and the night, with higher effect during the day. In addition, metergoline effectively attenuated the effects of serotonin. These facts suggest that in the cricket's optic lobe, serotonin is released during the daytime and sets the day state in the neurons regulating coupling between the bilaterally paired optic lobe circadian pacemakers.